Direct PCR

TransDirect^® Plant Tissue PCR Kit uses a unique lysis buffer to lyse plant tissues (fresh or frozen). The resulting lysate without purification can be directly used as PCR template. 2×TransDirect^® PCR SuperMix (+dye) is highly resistant to various PCR inhibitors present in plant tissues. PCR product can be directly used for gel electrophoresis.
 

• No DNA extraction needed.
• Ready-to-use SuperMix.
• DNA fragment up to 2 kb.
• For plants with moderate contents of polysaccharides or polyphenols.
 

Applications
• Direct amplification from unpurified lysate. Suitable for high throughput applications.
• Amplification of genomic DNA fragment up to 2 kb.
 

Storage

2×TransDirect® PCR SuperMix (+dye) at -20°C for two years, others at room temperature (15-25°C) for two years.

 

Shipping
2×TransDirect® PCR SuperMix (+dye) on dry ice (-70°C); others at room temperature.

 

Product Contents

Component	AD301-01	AD301-02
PD1 Buffer	4 ml	20 ml
PD2 Buffer	4 ml	20 ml
2×TransDirect® PCR SuperMix (+dye)	1 ml	5×1 ml
Nuclease-free Water	5 ml	25 ml