Expression Competent Cells

TransB(DE3) Chemically Competent Cell is specifically designed for chemical transformation of DNA. It is resistant to kanamycin (Kan^R) and tetracycline (Tet^R) and permits a transformation efficiency of over 10⁷ cfu/μg DNA (tested by pUC19 plasmid DNA).

Genotype
F^- ompT hsdSB(r_B^-m_B^-) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB (Kan^R,Tet^R)

Features
• Transformation efficiency: >10⁷ cfu/μg (pUC19 DNA).
• Kan^R and Tet^R.
• Thioredoxin reductase (trxB) and glutathione reductase (gor) mutation greatly facilitates cytoplasmic disulfide bond formation.
• Control plasmid I (Amp⁺) is used for detection of expression function of cell. The protein size is about 25 kDa.
 

Storage
at -70°C for six months. Do not store in liquid nitrogen.

Shipping
Dry ice (-70°C)