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Synthetic Defined Media
Synthetic Minimal Media (SD media) are based upon Yeast Nitrogen Base without Amino Acids and supplemented with a carbon source like glucose, galactose, raffinose, succinate or a combination of carbon sources.
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Overview
Synthetic Minimal Media (SD media) are based upon Yeast Nitrogen Base without Amino Acids and supplemented with a carbon source like glucose, galactose, raffinose, succinate or a combination of carbon sources. The Synthetic Minimal Media are available with or without Agar premixed.
Technical Data
|
|
SD Agar / Glucose |
SD Broth / Glucose |
SD Agar / Glucose w/o Phosphate |
SD Broth / Glucose w/o Phosphate |
SD Agar / Galactose |
SD Broth / Galactose |
SD Agar / Raffinose |
SD Broth / Raffinose |
SD Agar / Succinate |
SD Broth / Succinate |
SD Agar / Galactose and Raffinose |
SD Broth / Galactose and Raffinose |
|
Nitrogen Source g/l |
|
|
|
|
|
|
|
|
|
|
|
|
|
Ammonium Sulphate |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
|
Carbon Source g/l |
|
|
|
|
|
|
|
|
|
|
|
|
|
Glucose.H2O |
20 |
20 |
20 |
20 |
– |
– |
– |
– |
– |
– |
– |
– |
|
Galactose |
– |
– |
– |
– |
20 |
20 |
– |
– |
– |
– |
20 |
20 |
|
Raffinose |
– |
– |
– |
– |
– |
– |
20 |
20 |
– |
– |
10 |
10 |
|
Succinate |
– |
– |
– |
– |
– |
– |
– |
– |
33 |
33 |
– |
– |
|
Vitamins µg/l |
|
|
|
|
|
|
|
|
|
|
|
|
|
Biotin |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
|
Ca-Panthotenate |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
|
Folic Acid |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
2 |
|
Inositol |
2000 |
2000 |
2000 |
2000 |
2000 |
2000 |
2000 |
2000 |
2000 |
2000 |
2000 |
2000 |
|
Nicotinic Acid |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
|
p-Aminobenzoic Acid |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
|
Pyridoxine HCl |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
|
Riboflavin |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
|
Thiamine HCl |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
|
Trace Elements µg/l |
|
|
|
|
|
|
|
|
|
|
|
|
|
Boric Acid |
500 |
500 |
500 |
500 |
500 |
500 |
500 |
500 |
500 |
500 |
500 |
500 |
|
Copper Sulfate |
40 |
40 |
40 |
40 |
40 |
40 |
40 |
40 |
40 |
40 |
40 |
40 |
|
Potsssium Iodide |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
|
Ferric Chloride |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
|
Manganese Sulfate |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
|
Sodium Molybdate |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
|
Zinc Sulfate |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
|
Minerals g/l |
|
|
|
|
|
|
|
|
|
|
|
|
|
KH2PO4 |
1 |
1 |
– |
– |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
|
Magnesium Sulphate.anh |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
|
Sodium Chloride |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
|
Calcium Chloride.anh |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
|
Agar g/l |
|
|
|
|
|
|
|
|
|
|
|
|
|
Agar |
18 |
– |
18 |
– |
18 |
– |
18 |
– |
18 |
– |
18 |
– |
Product Range
- SD Agar / 2% Glucose
- SD Broth / 2% Glucose
- SD Agar / 2% Glucose w/o Phosphate (KH2PO4)
- SD Broth / 2% Glucose w/o Phosphate (KH2PO4)
- SD Agar / 2% Galactose
- SD Broth / 2% Galactose
- SD Agar / 2% Raffinose
- SD Broth / 2% Raffinose
- SD Agar / 3.3% Succinate
- SD Broth / 3.3% Succinate
- SD Agar / 2% Galactose / 1% Raffinose
- SD Broth / 2% Galactose / 1% Raffinose
For more details, kindly refer to the "Downloads" tab.
