In Vivo Imaging Solutions

Overview

The MOIS Series (HTP / HT / HTX) delivers high-performance in vivo imaging for small animal studies across oncology, immunology, pharmacology, and stem cell research.

With a back-illuminated CCD (≥90% quantum efficiency) and cooling capacity up to -90°C, MOIS ensures exceptional sensitivity for low-light applications. The fluorescence module features up to 26 narrowband filters and advanced spectral unmixing algorithms, improving multi-color accuracy and quantification.

Supporting a range of imaging fields from 2.5 × 2.5 cm to 25 × 25 cm, the system adapts to localized or whole-body needs. Built-in correction technologies—including bias, flat-field, background suppression, and cosmic ray removal—ensure consistent, reproducible results, ideal for high-standard in vivo research workflows.

Applications

• Cancer

• Drug Development

• Immunity & Stem Cells

• Developmental Biology

• Neuroscience

• Genetics & Molecular Biology

Highlights Of MOIS HT small animal in vivo Imaging System

• Equipped with a back-illuminated, -90°C depth-cooled CCD camera to effectively reduce background noise.

• Features a quantum efficiency of > 90%, with special coating processing on the chip to ensure high sensitivity in the 500-700nm range.

• F/Stop≤ 0.95.Lens collects more photons per unit time, capturing finer details and less noise.

• Covers a wide wavelength range of 400-900 nm, suitable for variouscommonly used dyes and probes in scientific research scenarios.

• Includes standard equipment with 19 excitation filters and 7 emission narrow band filters, ensuring a pure signal to avoid color crosstalk and provide realistic data.

• The calibrated light source meets NIST standards for absolutebioluminescence quantification, ensuring consistent results across imaging parameters.

• Excitation light intensity uniformity guarantees consistent strength across various fields of view, enhancing the reliability of fluorescence data collection.

• Field of view (FOV) ≥ 25cm × 25cm, enables simultaneous monitoring of at least 5 animals.

• Equipped with a 5-channel anesthesia tray and active scavenging masks to prevent anesthetic gas leakage.

Application Booklet

Specification parameters

Module	Parameters	MOIS HTP	MOIS HT	MOIS HTX
Imaging Modules	Fluorescence Imaging Module	√	√	√
	Bioluminescence Imaging Module	√	√	√
	Spectral High-Resolution Imaging	√	√	√
	X-Ray Module	×	×	√
	Upconversion Fluorescence Imaging (UCFI) Module	Expandable & Upgradeable
Detector	Camera Type	Back-illuminated sensor, Scientific-grade CCD
	Operating Temperature	-70℃	-90℃	-90℃
	Pixel Count	1024×1024
	Aperture (F-Stop)	≤ 0.95
	Minimum FOV (Field of View)	2.5cm × 2.5cm(optional)
	Maximum FOV	25cm × 25cm
	Quantum Efficiency	≥90%（500–700nm）
Laser System	Broadband Light Source	150W Halogen-tungsten broadband
	Guide Laser	Real-time FOV center display for positioning
Filters	Excitation Filters	19
	Emission Filters	7
Software	Real-time Acquisition System	Supports real-time image acquisition with light prompts, time series module, communication connection, and bioluminescence/fluorescence multi-mode imaging switching. Synchronous acquisition module supported.
	Offline Analysis Workstation	Support image analysis, time spectrum analysis module, need to be authorized by molecular software quantitative module
Environmental Control	Multi-Animal Imaging Chamber	5 channels
	Temperature Module	Temperature control range: 20-40 ℃, accuracy: ±0.1℃
	Anesthesia System	Anesthesia gas control: isoflurane, concentration: 0.5%-5% adjustable

Comparison of Small Animal In Vivo Imaging Systems: -70℃ vs -90℃

Model	MOIS HTP	MOIS HT
Parameter	-70℃ Version	-90℃ Version
Dark Current	0.0004 (typical) – 0.001 (max)	0.000177 (typical)
Readout Noise	@100KHz: 3.0 e⁻ (typical), 5 e⁻ (max) @2MHz: 9 e⁻ (typical), 15 e⁻ (max)	@50KHz: 2.9 e⁻ (typical) @1MHz: 6.6 e⁻ (typical)
Signal-to-Noise Ratio (SNR)	Slightly lower SNR, may affect high-precision imaging	Higher SNR, suitable for ultra-low noise high-resolution imaging
Image Quality	Good for standard imaging, less effective for low light signals	Excellent low light capture, ideal for high-resolution and low noise imaging
Application Scenarios	General small animal imaging experiments	High-precision imaging, low signal detection, long-term monitoring
Stability	Higher dark current and noise may affect result consistency	Lower dark current and noise enhance image stability and reliability
Research Suitability	Basic research and drug development	Preclinical research, oncology, drug tracking, and dynamic monitoring

Additional Recommendations:

• High-precision Imaging: The -90℃ version features significantly lower dark current and readout noise, ensuring sharper and more detailed images.
• Stability and Reliability: Lower dark current improves stability, crucial for long-term experiments.
• Future-Proof: The -90℃ version provides long-term value for increasingly precise imaging demands.
• Superior Low-Light Performance: Ideal for detecting weak light signals in fluorescence and molecular imaging.

Advantages

Ultra-High Sensitivity

Up to -90°C CCD cooling and ≥90% quantum efficiency enable exceptional low-light performance for bioluminescence and fluorescence imaging

Broad Spectral Compatibility

Excitation wavelengths from 400 to 900 nm support diverse fluorophores used in oncology, immunology, and stem cell research

Reliable Multimodal Imaging

Integrates bioluminescence, fluorescence, X-ray,and Cherenkov imaging in one system for versatile, in vivo experimental workflows.

Quantitative Accuracy

Calibration, spectral unmixing, and flat-field correction ensure reliable quantification across imaging sessions.

Scalable Field of View

Imaging range from 2.5 × 2.5 cm to 25 × 25 cm covers both localized and whole-body applications.

Data Consistency

Bias, background, and cosmic ray corrections enhance image uniformity and reproducibility.