Magnetofection

Overview

• High protein knockdown efficiency
• For all siRNA applications
• No off-target effects
• Serum compatible & Non toxic
• Simple, rapid and easy-to-use

• 200 µL: 400 assays in 96-well plate with 10nM siRNA
• 500 µL: 1000 assays in 96-well plate with 10nM siRNA
• 1 mL: 2000 assays in 96-well plate with 10nM siRNA
• 3 mL: 6000 assays in 96-well plate with 10nM siRNA
• #KC30300: siRNA Starting Kit with Super Magnetic plate + 200 µL of SilenceMag
• #KC30396 : siRNA Starting Kit with plate 96 magnets + 200 µL of SilenceMag
• #KC30400: Super Starting Kit with Super Magnetic plate + 100 µL of PolyMag, CombiMag, PolyMag Neo &  200 µL of SilenceMag
• #KC30496 : Super Starting Kit with plate 96 magnets + 100 µL of PolyMag, CombiMag, PolyMag Neo &  200 µL of SilenceMag

Storage: +4°C

Shipping Conditions: Room temperature

• This reagent needs to be used with a magnetic plate

Applications

• Ideal for gene silencing: siRNA, dsRNA, shRNA

• Suitable for mammalian cells: cell lines, primary cells, hard-to-transfect cells

• Perfect for all silencing applications:

  • sequential and simultaneous transfections

  • endogenous gene silencing

RECOMMENDED FOR: siRNA transfection of primary and hard-to-transfect cells.

Results

Figure 1: GFP silencing in HeLa cells. GFP-expressing HeLa cells seeded in a 96-well plate were transfected with 0.5 μL of SilenceMag and 4 or 10nM siRNA (corresponding to 10.8 or 27 ng respectively). GFP extinction was monitored 72h post-transfection by fluorescence microscopy.

Figure 2: LacZ silencing in various cell lines with SilenceMag. Various cells were co-transfected in 96-well plates with 100ng of pLacZ plasmid complexed to 0.1μL of PolyMag (#PN30100) and either 1 or 5nM of siRNA associated with SilenceMag. b-galactosidase expression was monitored after 48h using OZ Biosciencesbeta-Galactosidase assay kit (#OG10001).