Magnetofection

Overview

Magnetofection Transfection reagent - for a superior nucleic acid delivery in primary and hard-to-transfect cells

PolyMag Neo Magnetofection™ Reagent is specifically designed to achieve high transfection efficiency combined with superior transgene expression level. It is composed of magnetic nanoparticles coated with cationic molecules. PolyMag Neo is the ideal transfection reagent for a wide variety of cells.

• High transgene expression
• High transfection efficiency
• Multipurpose (various types of nucleic acids)
• Universal (primary cells and cell lines)
• Simple, ready-to-use and rapid
• Non toxic
• Compatible with and without serum-containing culture media

Sizes:

• 100 µL: 100 transfections with 1µg of DNA
• 200 µL: 200 transfections with 1µg of DNA
• 1000 µL: 1000 transfections with 1µg of DNA
• #KC30200 : Starting Kit with Super Magnetic plate + 100 µL of PolyMag Neo, PolyMag + CombiMag
• #KC30296 : Starting Kit with plate 96 magnets + 100 µL of PolyMag Neo, PolyMag + CombiMag
• #KC30400: Super Starting Kit with Super Magnetic plate + 100 µL of PolyMag, CombiMag, PolyMag Neo &  200 µL of SilenceMag
• #KC30496 : Super Starting Kit with plate 96 magnets + 100 µL of PolyMag, CombiMag, PolyMag Neo &  200 µL of SilenceMag

Storage: +4°C

Shipping conditions: Room temperature

• This reagent needs to be used with a magnetic plate

CATALOG NUMBER	UNIT SIZE
PG60100	100 µL
PG60200	200 µL
PG61000	1 mL
KC30200	Starting Kit
KC30296	Starting kit plate 96 magnets
KC30496	Super Starting Kit plate 96 magnets
KC30400	Super starting kit with Super Magnetic Plate

 

Applications

• Suitable for all nucleic acids delivery: DNA, oligonucleotides, mRNA, siRNA, shRNA...
• Improve your transgene expression experiments
• Perfect for all transfections: Transient, stable, gene silencing, with or without serum

RECOMMENDED FOR: Transfection of primary and hard-to-transfect adherent cells

 

Results

Figure 1: PolyMag Neo transfection efficiency in various cell lines. 1x10⁵ cells were transfected with 0.5 μg / well of pEGFP plasmid DNA in 24-well plates. Transfections were performed with 0.5 μL / well of polyMag Neo reagent. Percentage of transfected cells was measured 24h post transfection by flow cytometry.

Figure 2: PolyMag Neo transfection efficiency in various cell lines.1x10⁵ cells were transfected with 0.5 μg / well of pEGFP plasmid and 0.5 μL of PolyMag Neo reagent in 24-well plates. EGFP expression was monitored 24h after transfection by fluorescence microscopy.