qPCR and qRT-PCR SuperMixes

The kit contains a PerfectStart^® Taq DNA Polymerase, optimized dual-cation buffer, SYBR Green I, dNTPs, PCR enhancer and  PCR stabilizer. Three monoclonal antibodies bind to the hot-start Taq DNA Polymerase, effectively blocking DNA polymerase  activity and inhibiting non-specific amplification at low temperature. qPCR SuperMix is provided at 2×concentration and can be  used at 1×concentration by adding template, primers, Universal Passive Reference Dye and nuclease-free water.  

 

Highlights 

• Blocking by 3 antibodies; high specificity, sensitivity and amplification efficiency; applicable to a wide range of species.  

• Dual-cation buffer enhances specificity and reduces primer-dimer formation.  

• Universal Passive Reference Dye for different instruments to correct differences in fluorescence detection between wells due to  pipetting errors.

 

Storage
at -20°C  in dark for two years
 

Shipping
Dry ice (-70 ℃)

Product Composition

Component	AQ601-01-V2	AQ601-02-V2	AQ601-03-V2	AQ601-04-V2
2×PerfectStart® Green qPCR SuperMix	1 ml	5 x 1 ml	15 x 1 ml	25 x 1 ml
Universal Passive Reference Dye (50×)	40 μl	200 μl	600 μl	1 ml
Nuclease-free Water	1 ml	5 ml	3 x 5 ml	5 x 5 ml