Magnetofection

Overview

Magnetofectamine™ O2 is the new powerful and ideal transfection kit designed for primary and hard-to-transfect cells. The alliance of MTX transfection reagent and CombiMag leads to increased transfection efficiency, minimized toxicity and enhanced gene expression.

This kit replaces and outperforms the original Magnetofectamine™ which was the combination of Lipofectamine™* 2000 from Life Technologies Corporation and CombiMag Transfection Reagent.

CombiMag, a magnetic formulation based on the Magnetofection™ technology, binds to MTX transfection reagent/DNA complexes and under the application of a magnetic field concentrates the genetic material onto cells and promotes cellular uptake. In this way, transfection efficiency is enhanced.

• Less nucleic acids used - minimized toxicity
• No need to change your standard protocol
• Ideal for hard-to-transfect and primary cells
• Simple, ready-to use and rapid
• Serum compatible and applicable to all nucleic acids

Magnetofectamine O2 Transfection Kit for Primary Cells

Sizes :

• MTX2-1000 Starting Kit: 1 super Magnetic Plate (MF1000) + 250 µL CombiMag + 750 µL MTX reagent + 3 ml MTX Boost 100X
• MTX2-0750: 250 µL CombiMag + 750 µL MTX reagent + 3 ml MTX Boost 100X

Storage : Upon reception and for long-term use, store the MTX REAGENT and MTXBoost at -20°C and CombiMag at +4°C.

Shipping Conditions : Room temperature

*Lipofectamine™ and Invitrogen™ are Trademarks owned by Life Technologies Corporation.

• This reagent needs to be used with a magnetic plate

CATALOG NUMBER	UNIT SIZE
MTX2-0750	250 µL + 750 µL
MTX2-1000	Starting Kit

 

Applications

Magnetofectamine™ O2 is ideal for transfecting Primary cells as well as cell lines.

 

Results

Figure 1: Magnetofectamine O2 transfection efficiency.

 

Figure 2:  As the magnetic force drives the gene vector towards the target cells, Magnetofectamine™ O2 allows the vector dose to concentrate onto the cell very rapidly and triggers delivery via endocytosis. Consequently, high transfection efficiencies can be achieved with less nucleic acid amount.