Magnetofection

Overview

LipoMag Transfection Kit associates our most efficient lipid-based transfection reagent, DreamFect Gold with CombiMag magnetic nanoparticles, the most versatile Magnetofection™ reagent. By taking advantage of the Lipofection and Magnetofection transfection techniques, LipoMag Kit reaches even higher transfection efficiency especially in primary cells while reducing toxicity. It is suitable for all type of nucleic acids, including: plasmid DNA, linearized DNA, siRNA, oligonucleotides, double stranded RNA, mRNA, shRNA.

• Highest efficiency without toxicity

• Superior transgene expression level than any other reagents

• Enhancement of DreamFect™ Gold efficiency and outperformed competitors

• Biodegradable

• Multipurpose (various types of nucleic acids)

• Universal (cell lines and primary cells)

• Simple, Ready-to-use and Rapid

• Serum compatible  

Sizes:

• CombiMag 250µL + DreamFect Gold 500µL
• CombiMag 500µL + DreamFect Gold 1 mL

• Starting Kit: Super Magnetic Plate + 250µL of CombiMag, 500µL of DreamFect Gold

LipoMag Kit contains Dreamfect Gold transfection reagent + CombiMag transfection reagent

Storage: DreamFect Gold -20°C / CombiMag + 4°C

Shipping conditions: Room temperature

CATALOG NUMBER	UNIT SIZE
LM80250	250 µL + 500 µL
LM80500	500 µL + 1 mL
LM80000	Starting Kit

 

Applications

• Perfect for all transfection applications on primary and hard-to-transfect cells:

  • Gene expression

  • gene silencing

  • co-transfection

  • reverse transfection

• Suitable for all nucleic acids: DNA, siRNA, oligonucleotides, mRNA, shRNA...

RECOMMENDED FOR: Transfection of primary cells and hard-to-transfect cells

 

Results

Figure 1: BEAS-2B cells (1x10⁵ cells/ well) were transfected with Reagent L, DreamFect™ Gold or with LipoMag Kit in a 24-well plate using 0.5μg of GFP plasmid DNA [pVectOZ-GFP]. GFP positive cells were monitored by Flow Cytometry 24h after transfection.

 

Figure 2: A549 cells (1x10⁵ cells/well) were transfected with various amount of DNA in a 24-well plate. GFP positive cells were monitored 24 h post transfection by Flow Cytometry.