Reporter Gene mRNA

Overview

GFP mRNA has been designed to produce high expression level of Green Fluorescent Protein. It can be used as control of transfection efficiency. 

OZ Biosciences mRNAs are produced by in vitro transcription and stabilized at the 5’ end by modified nucleotides capping (Cap1). These mature mRNAs contain a poly(A) tail at the 3’ end and are also optimized to yield improved stability and performance.

GFP mRNAs:

• #MRNA15 does not bear any additional nucleotide modifications.
• #MRNA11 is modified with 5-methoxyuridine (5moU) to reduce innate immune response.
• #MRNA22 is modified with N1-methyl-pseudouridine (N1-mψ) to reduce innate immune response.
• #MRNA15c and #MRNA11c are labelled with Cy5 (Sulfo-Cyanine5) by replacing 20 % of U or moU by UTP-Cy5.

 

We recommend using our transfection reagents RmesFect or RmesFect Stem in order to reach high transfection efficiency and optimum protein expression level.

 

Size: 20 µg, 100µg and 1 mg

Storage: -80°C

mRNAs ARE SHIPPED WITH DRY ICE

 

CATALOG NUMBER	UNIT SIZE
MRNA15-20	mRNA GFP 20 µg
MRNA15-100	mRNA GFP 100µg
MRNA15-1000	mRNA GFP 1 mg
MRNA11-20	mRNA GFP (5moU) 20 µg
MRNA11-100	mRNA GFP (5moU) 100 µg
MRNA11-1000	mRNA GFP (5moU) 1mg
MRNA15C-100	GFP mRNA (U-Cy5) 100 µg
MRNA11C-100	GFP mRNA (5moU/U-Cy5) 100 µg
MRNA22-20	GFP mRNA (N1-mψ) 20 µg
MRNA22-100	GFP mRNA (N1-mψ) 100 µg
MRNA22-1000	GFP mRNA (N1-mψ) 1 mg

 

Applications 

GFP mRNA has been designed to produce high expression level of Green Fluorescent Protein. It is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

 

Results

Figure 1: Jurkat T cells, Raw264.7, DC2.4, primary human Adipose Stem Cells, pri-mary human Mesenchymal Stem Cells and Mouse Embryonic Fibroblasts were transfected using RmesFect transfection reagent and mRNA-GFP from OZ Biosciences.