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Overview
Overview
Genome EditorTM is a simple and easy-to-use electroporator suitable for zygote genome editing using CRISPR/Cas9 system.
Application
High throughput and effcient CRISPR/Cas9-based mouse genome editing by RNA electroporation to zygote.
Fgf10 gene knockout mouse by Cas9 mRNA/gRNA electroporation
Fgf10 homozygous mutant embryo have an easily detectable limbless phonotype. Electroporation of Cas9 mRNA (Cas9 Protein) and gRNA enables genome editing of Fgf10.
Electrodes for zygote genome editing
Platinum plate electrode on tempered glass, LF501PT1-10, can hold up to ~40 mouse zygotes in a 5 μL electroporation buffer and enables efficient and high throughput zygote genome editing using CRISPR/Cas9 by electroporation.
Electroporation vs Microinjection
|
|
Electroporation |
Microinjection |
|---|---|---|
|
Pre-operation |
Not necessary |
Preparation of injection and hold pippettes, etc.
|
|
Required time |
~5 minutes |
> 2 hours |
|
Viability (after E15) |
40-80% |
10-50% |
|
Efficiency |
Same as microinjection |
Depends on the sequence |
|
Required skills |
Not necessary |
Maniipularion of single zygotes |
|
Required amount of Cas9 mRNA |
500 - 2,000 ng |
50 - 500 ng |
More zygotes can be treated at one time by electroporation than microinjection.
Viability of treated zygotes are much higher in electroporation than in microinjection.
No special skills are required for electroporation compared to microinjection, which requires injection technique that is time-consuming to acquire.
Electroporation is, therefore, the ideal method for high throughput mouse zygote genome editing by CRISPR/Cas9 system.
Specifications
|
Voltage |
1 - 200 V / 1 V resolution |
|---|---|
|
Max current |
1000 mA |
|
Pulse length (Pon) |
0.1 - 1000 ms |
|
Pulse interval (Poff) |
1.00 - 1000 ms |
|
Pulse number |
1 - 1000 for Pd(+) |
|
Impedance measurement |
Up to 4.00 kΩ |
|
Voltage measurement |
-200 V - +200 V / 1 V resolution |
|
Current measurement |
-1023 mA - +1024 mA / 1 mA resolution |
|
Memorable programs |
>20000 programs |
|
History |
Last 100 histories are saved. |
|
Power |
100 - 115 or 220 V, 50/60 Hz |
|
Fuse |
10 A (6.3 mm x 20 mm) |
|
Dimensions |
W 240 mm X L 380 mm X H 190 mm (w/o rubber stand, umbo) |
|
Weight |
5.5 kg |
