Magnetofection

OZ Biosciences has created fluorescently-labeled magnetic nanoparticles for Magnetofection applications. FluoMag Transfection Reagents are as efficient as their non-labeled counterparts and allow you to visualize the nanoparticles in vitro during your experiments.

Sizes : 100 µL

Storage: +4°C and protect from the light

Shipping Conditions: Room temperature

CATALOG NUMBER	UNIT SIZE
FN10100	FluoMag-N 100 µL
FC10100	FluoMag-C 100µL
FP10100	FluoMag-P 100µL
FS10100	FluoMag -S 100µL
FV10100	FluoMag-V 100µL

 

Results

Figure 1: NIH-3T3 transfection with FluoMag-P and pEGFP plasmid DNA. NIH-3T3 cells (5x10⁴ cells/well), growing on coverslips, were transfected in 24-well plates with 1 µg of pEGFP plasmid and 1µL of  FluoMag-P per well as described in the Magnetofection instruction manual. At several times post-transfection, cells were fixed, stained with DAPI to detect nucleus (blue) and observed under a fluorescent microscope equipped with a CCD camera.

Figure 2: Transfection efficiency of FluoMag-S versus SilenceMag. GFP stably transfected MDCK and HeLa cells were plated the day before transfection in a 24-well plate. Cells were then treated with SilenceMag or FluoMag-S and siRNA (targeting GFP or targeting LacZ as control) as described in the SilenceMag instruction manual. Complexes were prepared with 1 μL of SilenceMag or FluoMag-S and 10nM (67.5ng) of siRNA. Cells were then transfected in 500 μL transfection volume. GFP expression level was monitored 72h post-transfection by detection of fluorescence intensity with a fluorometer.