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Overview
Ready-to-use stabilized F-Luc mRNA
Concentration: 1.0 mg/mL in 1 mM Sodium Citrate, pH 6.4
F-Luc mRNA has been designed to produce high expression level of FireFly Luciferase. It is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.
OZ Biosciences mRNAs are produced by in vitro transcription and stabilized at the 5’ end by modified nucleotides capping (Cap1). These mature mRNAs contain a poly(A) tail at the 3’ end and are also optimized to yield improved stability and performance.
F-Luc mRNAs:
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#MRNA16 does not bear any additional nucleotide modifications.
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#MRNA12 is modified with 5-methoxyuridine (5moU) to reduce innate immune response.
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#MRNA24 is modified with N1-methyl-pseudouridine (N1-mψ) to reduce innate immune response.
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#MRNA12c and #MRNA16c are labelled with Cy5 (Sulfo-Cyanine5) by replacing 20 % of U or moU by UTP-Cy5.
We recommend using our transfection reagents RmesFect or RmesFect Stem in order to reach high transfection efficiency and optimum protein expression level.
Size: 20 µg, 100µg and 1 mg
Storage: -80°C
mRNAs ARE SHIPPED WITH DRY ICE
| CATALOG NUMBER | UNIT SIZE |
| MRNA16-20 |
F-Luc mRNA 20 µg |
| MRNA16-100 |
F-Luc mRNA 100 µg |
| MRNA16-1000 |
F-Luc mRNA 1mg |
| MRNA12-20 |
mRNA F-Luc (5moU) 20 µg |
| MRNA12-100 |
mRNA F-Luc (5moU) 100 µg |
| MRNA12-1000 |
mRNA F-Luc (5moU) 1mg |
| MRNA16C-100 |
F-Luc mRNA (U-Cy5) 100 µg |
| MRNA12C-100 |
F-Luc mRNA (5moU/U-Cy5) 100 µg |
| MRNA24-20 |
F-Luc mRNA (N1-mψ) 20 µg |
| MRNA24-100 |
F-Luc mRNA (N1-mψ) 100 µg |
| MRNA24-1000 |
F-Luc mRNA (N1-mψ) 1 mg |
