PCR Enzymes

EasyTaq^® DNA Polymerase for PAGE is purified from E. coliexpressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq^® DNA Polymerase for PAGE has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. lt lacks 3′-5′ exonuclease activity. This enzyme is supplied with unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.
 

• Extension rate is about 1-2 kb/min.
• Unique buffer system compatible with PAGE.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY^®-T vectors.
• Amplification of genomic DNA fragment up to 3 kb.
 

Applications
Short fragment PCR
 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AP112-01/11	AP112-02/12
EasyTaq® DNA Polymerase for PAGE	2500 U	2500 U×4
10×EasyTaq® Buffer for PAGE	1.2 ml ×5	1.2 ml×20
2.5 mM dNTPs	- / 800 μl×5	- / 800 μl×20
6×DNA Loading Buffer	1 ml	1 ml ×4