RT-PCR

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EasyScript® Reverse Transcriptase

EasyScript® Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity.

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Overview

EasyScript® Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity. The enzyme is purified to near homogeneity from E.coli containing the modified M-MLV RT gene.

• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
• cDNA up to 8 kb.
 

Applications
• First-strand cDNA synthesis
• Multiple copy gene detection
 

Storage
at -20°C for two years

Shipping
Dry ice (-70°C)

 

Product Contents
Component AE101-02 AE101-03
EasyScript® RT 10000 U 5×10000 U
5×ES RT Buffer 200 μl 5×200 μl
Anchored Oligo(dT)18 Primer (0.5 μg/μl) 50 μl 250 μl