RT-PCR

EasyScript^® First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by EasyScript^® RT/RI Enzyme Mix and 2×ES Reaction Mix.

• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• The product obtained from 15 minutes reaction is used for qPCR; the, product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)18 Primer is specifically designed to bind to the first base next to mRNA Poly(A)⁺ on the 5' end with high specificity, ensuring high efficiency and success rate of first-strand cDNA synthesis.
• Random Primer (N9) or Gene Specific Primer (GSP) can be used to synthesize the first-strand cDNA.
• cDNA up to 8 kb.
 

Applications
Multiple copy gene detection
 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AE301-02	AE301-03
EasyScript® RT/RI Enzyme Mix	50 μl	100 μl
2×ES Reaction Mix	500 μl	1 ml
Random Primer(N9) (0.1 μg/μl)	50 μl	100 μl
Anchored Oligo(dT)18 Primer (0.5 μg/μl)	50 μl	100 μl
RNase-free Water	500 μl	1 ml