RT-PCR

5×EasyScript^® All-in-One SuperMix for qPCR provides all the necessary reagents for cDNA synthesis from total RNA or mRNA (including 5×EasyScript^® RT, RNase Inhibitor, Anchored Oligo (dT)₁₈ Primer, Random Primer (N9), dNTPs, Buffer). It is provided at 5× concentration and used at 1× concentration by only adding gDNA remover, RNA template and H₂O for efficient first-strand cDNA synthesis. Simultaneously, residual genomic DNA from RNA template can be removed. 5×EasyScript^® All-in-One No-RT Control SuperMix for qPCR is supplied to prepare no-reverse transcriptase (RT) control, which is used to assess if the qPCR template is contaminated with genomic DNA. This product is capable of minimizing contamination during operation with a simple workflow. The resulting cDNA is only suitable for qPCR, not for regular PCR.

 

Highlights

• “All-in-One SuperMix” type: Simultaneous cDNA synthesis and genomic DNA removal by only adding gDNA remover, RNA template and H₂O

• High synthesis efficiency enabled by optimal ratio of Oligo(dT)₁₈ Primer to Random Primer (N9) and optimized composition of the SuperMix, ensuring same reverse transcription efficiency for RNA templates of different concentrations and specifically high synthesis efficiency for short cDNA.

• Only 15 minutes for reverse transcription.

• High compatibility with qPCR reagents.

 

Applications
High-copy-number and low-copy number gene detection

 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AE341-02 (100 rxns)	AE341-03 (500 rxns)
5×EasyScript® All-in-One SuperMix for qPCR	400 μl	5×400 μl
5×EasyScript® All-in-One No-RT Control SuperMix for qPCR	40 μl	200 μl
gDNA Remover	100 μl	5×100 μl
Nuclease-free Water	2×1 ml	2×5 ml