Plasmid DNA Purification

EasyPure^® Plasmid MiniPrep Kit uses a modified alkaline lysis method to isolate high-quality plasmid DNA from ≤20 ml (LB) of bacterial culture. Unique formulated lysis buffer and neutralization buffer permit complete bacterial cell lysis and neutralization. The purified plasmid DNA is suitable for a variety of molecular biology applications, including restriction enzyme digestion, ligation, transformation and DNA sequencing.

 

• Simple and fast: the whole procedure can be performed in 20 minutes.
• High yield: DNA yield up to 40 μg.
 

Storage
RNase A at -20°C for two years; others at room temperature (15-25°C) in a dry place for one year. 

 

Shipping
RNase A on dry ice (-70°C); others at room temperature.

 

Product Contents

Component	EM101-01 (50 rxns)	EM101-02 (200 rxns)
Resuspension Buffer (RB)	15 ml	60 ml
Lysis Buffer (LB)	15 ml	60 ml
Neutralization Buffer (NB)	20 ml	80 ml
Wash Buffer (WB)	10 ml	2×20 ml
Elution Buffer (EB)	5 ml	10 ml
RNase A (10 mg/ml)	150 μl	600 μl
Mini-Plasmid Spin Column with Collection Tubes	50 each	2×100 each