Plasmid DNA Purification

EasyPure^® HiPure Plasmid MiniPrep Kit provides an efficient way to isolate high yield (up to 40 μg) and high quality plasmid DNA from ≤20 ml (LB) of bacterial culture. Unique formulated lysis buffer and neutralization buffer permit complete bacterial cell lysis and neutralization.Endotoxin is removed by a simple incubation on column with a novel buffer. The purified plasmid DNA is suitable for a variety of molecular biology applications, including restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection.
 

• Fast: the whole procedure can be performed in 20 minutes.
• Simple: endotoxin is removed on column.
• High yield: DNA yield up to 40 μg.
 

Storage
RNase A at -20°C for two years; others at room temperature (15-25°C) for one year.
 

Shipping
RNase A on dry ice (-70 °C); others at room temperature.

 

Product Contents

Component	EM111-01 (50 rxns)
Resuspension Buffer (RB)	15 ml
Lysis Buffer (LB)	15 ml
Neutralization Buffer (NB)	20 ml
ToxinOut Buffer (TB)	15 ml
Wash Buffer (WB)	10 ml
Elution Buffer (EB)	5 ml
RNase A (10 mg/ml)	150 μl
Mini-Plasmid Spin Columns with Collection Tubes	50 each