Plasmid DNA Purification

EasyPure^® HiPure Plasmid MaxiPrep Kit uses a modified alkaline lysis method to isolate high quality plasmid DNA from ≤ 500 ml (LB) of bacterial culture. Unique formulated lysis buffer and neutralization buffer permit complete bacterial cell lysis and neutralization. Endotoxin is removed by a simple incubation on column with a novel buffer. The purified DNA is suitable for a variety of molecular biology applications including restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection.
 

• Fast: the whole procedure can be performed in one hour.
• Simple: endotoxin is removed on column.
• High yield: DNA yield up to 1 mg.
 

Storage
RNase A at -20°C for two years; others at room temperature (15-25°C) for one year.

Shipping
RNase A on dry ice (-70 °C); others at room temperature.

 

Product Contents

Component	EM121-01 (10 rxns)
Resuspension Buffer (RB)	120 ml
Lysis Buffer (LB)	120 ml
Neutralization Buffer (NB)	160 ml
ToxinOut Buffer (TB)	60 ml
Wash Buffer (WB)	25 ml
Elution Buffer (EB)	25 ml
RNase A (10 mg/ml)	1.2 ml
Maxi-Plasmid Spin Columns with Collection Tubes	10 each