Genomic DNA Purification

EasyPure^® Genomic DNA Kit provides a simple and convenient way to efficiently isolate high-quality genomic DNA from a variety of materials (mammalian cells, tissues, mouse tails, E. coli and yeast). Cells and tissues are enzymatically lysed. DNA is specifically bound to silica-based column. The isolated DNA is suitable for PCR, restriction enzyme digestion and Southern blot, etc.

• Fast extraction, high yield (up to 15 μg).

• High integrity of cDNA ensured by mild lysis conditions without physical disruption, thus reducing damage to gDNA during cell lysis process.

• High purity enabled by spin column which can efficiently and specifically bind to DNA and removs protein, salts, lipids or other contaminants.

 

Storage
RNase A at -20°C for two years; others at room temperature (15-25°C) in a dry place for one year.

 

Shipping
RNase A on dry ice (-70 °C); Others at room temperature.

Product Contents

 

Component	EE101-01 (50 rxns)	EE101-02 (200 rxns)	EE101-11 (50 rxns)	EE101-12 (200 rxns)
Lysis Buffer 2 (LB2)	6 ml	24 ml	6 ml	24 ml
Binding Buffer 2 (BB2)	28 ml	110 ml	28 ml	110 ml
Clean Buffer 2 (CB2)	15 ml	60 ml	15 ml	60 ml
Wash Buffer 2 (WB2)	12 ml	2×22 ml	12 ml	2×22 ml
Elution Buffer (EB)	25 ml	80 ml	25 ml	80 ml
RNase A (20 mg/ml)	1 ml	4×1 ml	0	0
Proteinase K (20 mg/ml)	1 ml	4×1 ml	1 ml	4×1 ml
Genomic Spin Column with Collection Tubes	50 each	200 each	50 each	200 each