RNA Purification

EasyPure^® Blood RNA Kit provides a simple and fast column based method to isolate total RNA from 50 μl-1.5 ml of fresh or anticoagulated blood. Blood is lysed and DNA is digested with DNase I. RNA is bound to silica membrane. After washing, high quality RNA is eluted. Purified RNA is suitable for RT-PCR, qRT-PCR and Northern blot.
 

Storage

DNase I and DNase I Reaction Buffer at -20°C for one year; others at room temperature (15-25°C) in a dry place for one year.

 

Shipping
DNase I and DNase I Reaction Buffer on dry ice (-70°C); others at room temperature.

 

Product Contents

Component	ER401-01 (50 rxns)
Red Cell Lysis Buffer 2 (RCL2)	125 ml
Binding Buffer 7 (BB7)	40 ml
Clean Buffer 7 (CB7)	60 ml
Wash Buffer 7 (WB7)	12 ml
DNase I (3 units/μl)	1500 units
DNase I Reaction Buffer	4×1 ml
RNase-free Water	10 ml
RNA Spin Columns with Collection Tubes	50 each
RNase-free Tube (1.5 ml)	50 each