Genomic DNA Purification

EasyPure^® Blood Genomic DNA Kit provides a simple and convenient way to isolate high quality genomic DNA from 5-250 μl of fresh or frozen blood. Whole blood is incubated with binding/lysis buffer to release DNA. DNA is bound to silica-based column.

• Simple and fast, red cell lysis buffer is no longer needed.
• Complete removal of contaminants and inhibitors.
• DNA yield up to 40 μg.
• Column based purification, no organic extraction or ethanol precipitation.
• Suitable for EDTA, sodium citrate and heparin-anticoagulated fresh or frozen blood in a volume of 5 to 250 μl.
 

Applications
PCR, restriction enzyme digestion and Southern blot
 

Storage
At room temperature (15-25°C) in a dry place for one year; RNase A at -20°C for two years.
 

Shipping
RNase A on dry ice (-70 °C); others at room temperature.

Product Contents

 

Component	EE121-01 (50 rxns)	EE121-02(200 rxns)	EE121-11 (50 rxns)	EE121-12(200 rxns)
Binding Buffer 3 (BB3)	30 ml	110 ml	30 ml	110 ml
Clean Buffer 3 (CB3)	6 ml	24 ml	6 ml	24 ml
Wash Buffer 3 (WB3)	12 ml	2×22 ml	12 ml	2×22 ml
Elution Buffer (EB)	25 ml	80 ml	25 ml	80 ml
RNase A (20 mg/ml)	500 μl	2×1 ml	0	0
Proteinase K (20 mg/ml)	1 ml	4×1 ml	1 ml	4×1 ml
Genomic Spin Columns with Collection Tubes	50 each	200 each	50 each	200 each