Genomic DNA Purification

EasyPure^® Bacteria Genomic DNA Kit uses lysozyme and moderate lysis buffer to lyse cells. Proteinase K is used for protein digestion and RNase A used for RNA digestion. DNA is specifically bound to silica-based column in hypersaline condition, and DNA is eluted by low salt and high pH solution. This kit is suitable for isolating high quality genomic DNA from Gram-positive and Gram-negative bacteria. The isolated DNA is suitable for PCR, restriction enzyme digestion, and Southern blot.

• Fast: the whole process can be completed in 50 minutes.
• High yield: DNA yield up to 20 μg.
 

Applications
PCR, restriction enzyme digestion and Southern blot
 

Storage
RNase A at -20°C for two years; others at room temperature (15-25°C) for one year.

Shipping

RNase A on dry ice (-70°C); others at room temperature.

 

Product Contents

Component	EE161-01 (50 rxns)	EE161-11 (50 rxns)
Resuspension Buffer11 (RB11)	12 ml	12 ml
Lysis Buffer11 (LB11)	6 ml	6 ml
Binding Buffer11 (BB11)	10 ml	10 ml
Clean Buffer 11 (CB11)	55 ml	55 ml
Wash Buffer 11 (WB11)	12 ml	12 ml
Elution Buffer (EB)	25 ml	25 ml
RNase A (10 mg/ml)	1 ml	0
Proteinase K (20 mg/ml)	1 ml	1 ml
Genomic Spin Columns with Collection Tubes	50 each	50 each