Cell Isolation Nanobeads

Overview

Efficient and gentle cell isolation is crucial for researching specific cell populations and developing life-saving cell and gene therapy products. Purity, recovery, and viability are key characteristics post-isolation.

GenScript excels in developing magnetic bead products for biomolecule separation. CytoSinct™ magnetic beads use immunomagnetic cell separation technology, combining antibody-based purification specificity with nanoparticle gentleness.

The CytoSinct™ manual cell isolation platform includes antigen-specific paramagnetic nanobeads, a column, and a magnetic separator. These nanometer-sized, biodegradable matrix-coated beads are non-toxic, user-friendly, ensuring highly efficient cell isolation. CytoSinct™ columns amplify the magnetic field for effective separation with minimal labeling. Isolated cells are compatible with downstream applications like cell culture, activation, expansion, flow cytometry, and translational research.

With the flexibility and specificity of CytoSinct™, you can use various starting materials for enriching or depleting desired cell types. Each use guarantees high-purity, high-recovery isolated samples.

 

Product Highlights

• Highly efficient
• Biodegradable matrix
• Nontoxic
• Compatible
• Easy to use
• Sterilized
• Paramagnetic

 

Workflow

 

Highly Efficient CD4+CD8+T Cell Isolation from PBMCs

Purity, recovery, and viability are crucial characteristics of cells post-isolation. CytoSinct™ Nanobeads, being nanometer-sized, enable gentle and efficient isolation of target cells. This ensures that your post-isolation cells are suitable for a variety of downstream applications.

 

PBMCs were isolated using CytoSinct™ CD4 Nanobeads, human (RUO) (Cat# L00863) and CytoSinct™ CD8 Nanobeads, human (RUO) (Cat# L00864), theresults shows high purity(A,B), high recovery(C) and high viability(D).
 

Comparing to the similar products from the competitor, GenScript CytoSinct™ Nanobeads can provide the target cells with high purity and recovery rate.

Subpopulation phoenotype of post-isolation cells is important for functional and phenotypic studies. Non-T subpopulations like monocyte and NK cells could impact the efficacy of T-cells or therapeutic CAR-T and TCR-T generated from the enrichment. GenScript CytoSinctTM NanoBeads for CD4 and CD8 T-cell isolation efficiently minimize non-T composition in the enrichment population.

 

Highly Efficient T Cell Isolation from Apheresis

CD4+&CD8+ or CD3+ T cell isolation are both commonly used for the relevant T cell studies. GenScript can provide high quality of both RUO and GMP grade CytoSinct™ Nanobeads, which can support your studies from bench-side to bed-side.

Representative data comparing T cells isolation from leukopheresis samples using GenScript CytoSinctTM Nanobeads and competitor products.
A. Shows comparison of pre and post isolation data, purity, recovery, and viability for co-isolation of CD4 and CD8 T cell from apheresis using CytoSinctTM CD4 + CytoSinctTM CD8 and competitor reagent.
B. Shows comparison of pre and post isolation data, purity, recovery, and viability. CD3 T cell from apheresis using CytoSinctTM CD3 and competitor reagent.

Isolated T cells using CytoSinct™ Nanobeads has a high purity and recovery rate.

 

Enceed™ — World Leading T Cell Activation Reagent

GenScript Enceed™ T cell activation uses CD3 and CD28 as the activation marker for in vitro activation and expansion of human T cells. Comparing to the similar products from the competitor, activated T cells using Enceed™ can obtain higher expansion folds and larger total amount of TSCM+TCM, which is greatly beneficial for the downstream application.

(A) Isolated CD4+CD8+T cells were activated using the Enceed™ T cell activation reagent from GenScript, comparing with the similar activation product from the competitor. After the activation, the cell samples were cultured and expanded for 48 hours, and the CD25+ & CD69+ expression were detected by flowcytometry and shown.

(B) To show the expansion status after the activation, isolated T cells were stained with CFSE before and 7 days after the activation.

(C) The expansion folds of the activated T cells were shown along the course of 17 days with the comparison of the activated T cells using competitor's product.

(D) The memorial phenotypes of the activated T cells were monitored and shown on Day 12 and Day 17 after the activation.

 

CytoSinct™ Column and Magnetic Separator

CytoSinct™ Column 

• Anti-clogging property: Innovative structure
• Compatibility: compatible with beads from other vendors
• High performance: High purity, recovery and viability cells after separation

 

CytoSinct™ Magnetic Separator 

• High Recovery & Fast Isolation
• Flexible Throughput(single/multi-channel)
• Easy to operate

 

CytoSinctTM 1000 Automated Cell Isolation Instrument

Representative data of Cell Isolation with CytoSinct™ 1000 system

Group	CytoSinct™ Nanobeads	Positive cell%	Instrument	Tubing Set type
Competitor	CytoSinct™ CD4&CD8	64.40%	Competitor Instrument	Competitor Tubing Set
GenScript	CytoSinct™ CD4&CD8	64.40%	GenScript CytoSinct™ 1000	GenScript CytoSinct™ 1000 Tubing Set

 

• GenScript CytoSinct™ 1000 system can achieve a high purity, recovery rate and viability of the target cells.
• Higher cell recovery rate is observed, since CytoSinct™ 1000 instrument has a relevant higher elution efficiency.
• The closed and sterilized CytoSinct™ 1000 Tubing Set Products are optimized for different applications.

Learn more

 

Cell Therapy Product List

Cell Isolation Nanobeads

RUO grade Nanobeads

Catalog Number	Product Name	Package Size	Capacity
L00863-1	CytoSinct™ CD4 Nanobeads, human	1 mL	Up to 1X109 total MNCs
L00863-0.5	CytoSinct™ CD4 Nanobeads, human	500 uL	Up to 5X108 total MNCs
L00864-1	CytoSinct™ CD8 Nanobeads, human	1 mL	Up to 1X109 total MNCs
L00864-0.5	CytoSinct™ CD8 Nanobeads, human	500 uL	Up to 5X108 total MNCs
L00896-1	CytoSinct™ CD3 Nanobeads, human	1 mL	Up to 1X109 total MNCs
L00896-0.5	CytoSinct™ CD3 Nanobeads, human	500 uL	Up to 5X108 total MNCs
L00898-1	CytoSinct™ Streptavidin Nanobeads	1 mL	Up to 1X109 total MNCs
L00898-0.5	CytoSinct™ Streptavidin Nanobeads	500 uL	Up to 5X108 total MNCs
L00897-7.5 coming soon	CytoSinct™ TCR αβ Nanobeads, human	7.5 mL	Up to 2X1010 total MNCs
L00903-1	CytoSinct™ CD56 Nanobeads, human	1 mL	Up to 1X109 total MNCs
L00903-0.5	CytoSinct™ CD56 Nanobeads, human	500 uL	Up to 5X108 total MNCs
L00967-1	CytoSinct™ CD34 Nanobeads, human	1 mL	Up to 1X109 total MNCs
L00956 coming soon	CytoSinct™ CD14 Nanobeads, human	1 mL	Up to 1X109 total MNCs
L00957 coming soon	CytoSinct™ CD19 Nanobeads, human	1 mL	Up to 1X109 total MNCs
L00958 coming soon	CytoSinct™ CD25 Nanobeads, human	1 mL	Up to 1X109 total MNCs

 

GMP grade Nanobeads

Catalog Number	Product Name	Package Size	Capacity
L00932-2.5	CytoSinct™ CD4 Nanobeads, human (GMP)	2.5 mL	Up to 1.33X1010 total MNCs
L00932-7.5	CytoSinct™ CD4 Nanobeads, human (GMP)	7.5 mL	Up to 4X1010 total MNCs
L00933-2.5	CytoSinct™ CD8 Nanobeads, human (GMP)	2.5 mL	Up to 1.33X1010 total MNCs
L00933-7.5	CytoSinct™ CD8 Nanobeads, human (GMP)	7.5 mL	Up to 4X1010 total MNCs
L00934	CytoSinct™ CD3 Nanobeads, human (GMP)	7.5 mL	Up to 4X1010 total MNCs
L00948 coming soon	CytoSinct™ CD34 NanoBeads, human (GMP)	7.5 mL	Up to 4X1010 total MNCs
L00949 coming soon	CytoSinct™ CD56 NanoBeads, human (GMP)	7.5 mL	Up to 4X1010 total MNCs
L00952 coming soon	CytoSinct™ TCR αβ Nanobeads, human (GMP)	7.5 mL	Up to 2X1010 total MNCs

 

Enceed™ Activation Reagents

RUO grade Activation Reagent

Catalog Number	Product Name	Package Size	Capacity
L00899-1	Enceed™ T cell Activation, human	1 mL	Up to 2X108 T cells
L00899-0.5	Enceed™ T cell Activation, human	500 uL	Up to 1X108 T cells

 

GMP grade Activation Reagent

Catalog Number	Product Name	Package Size	Capacity
L00935	Enceed™ T cell Activation, human (GMP)	4 mL	Up to 2X108 T cells

 

Manual Separators

Catalog Number	Product Name	Package Size	Capacity
D00006	CytoSinct™ gStand	1 pc	Compatible with all CytoSinct™ Magnets
D00007	CytoSinct™ gM columns	25 pc	Up to 2×108 total cells (each column)
D00008	CytoSinct™ gL columns	25 pc	Up to 2×109 total cells (each column)
D00009	CytoSinct™ M1 magnet	1 pc	Holds 1 CytoSinct™ gM column
D00010	CytoSinct™ M8 magnet	1 pc	Holds 8 CytoSinct™ gM columns
D00011	CytoSinct™ L1 magnet	1 pc	Holds 1 CytoSinct™ gL column
D00012	CytoSinct™ L4 magnet	1 pc	Holds 4 CytoSinct™ gL columns

 

CytoSinct™1000 (Clinical Grade System)

Catalog Number	Product Name	Package Size	Capacity
D00023	CytoSinct™ 1000	1 unit	Compatible with CytoSinct™ 1000 tubing set products
D00029	CytoSinct™ 1000 Tubing Set	1 set	Up to 6×1010 total cells
D00030	CytoSinct™ 1000 LS Tubing Set	1 set	Up to 1.2×1011 total cells
D00031	CytoSinct™ 1000 Depletion Tubing Set	1 set	Up to 1.2×1011 total cells