Expression Competent Cells

BL21(DE3) Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 10⁷ cfu/μg DNA (tested by pUC19 plasmid DNA).

Genotype
F^- ompT hsdS(r_B^-m_B^-) gal dcm(DE3)

Features
• Transformation efficiency: >10⁷ cfu/μg (pUC19 DNA).
• DE3 strains contains the λDE3 lysogen that carries the gene for T7 RNA polymerase.
• Suitable for T7 and T7lac such as pET, pEASY^®.
• Suitable for high expression of non-toxic protein.
• Control plasmid I (Amp⁺) is used for detection of expression function of cell. The protein size is about 25 kDa.
 

Storage
at -70°C for six months. Do not store in liquid nitrogen.

Shipping
Dry ice (-70°C)