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AdenoMag Transduction Reagent

For Adenovirus and AAV transduction

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Overview

Overview

AdenoMag Transfection Reagent is a magnetic nanoparticles formulation specifically dedicated to enhance Adenovirus and Adeno Associated Virus (AAV) infection and transduction capacities. Based on the Magnetofection™ technology, it allows concentrating the complete applied dose of viral particles onto cells within minutes, inducing a significant improvement of virus infectivity with extremely low vector doses. Due to its specific properties, AdenoMag is applicable to all adenoviral and AAV vectors and is ideal to infect non permissive cells. This reagent demonstrates an exceptionally high efficiency to promote, control and assist Adenoviral and AAV transductions in a way that no molecular biology processes or biochemical modifications are required. 
  • Increase transduction efficiency
  • Accelerate the transduction process
  • Efficient for hard-to-infect and non-permissive cells
  • Concentrate the viral dose onto the cells
  • Synchronise cells adsorption/infection without modifications of the viruses
Sizes:
  • 100 µL: 500-100 transductions based on MOI of 1 for 104 cells per 96 wells
  • 200 µL: 1000-2000 transductions based on MOI of 1 for 104 cells per 96 wells
  • 1000 µL: 5000-10000 transductions based on MOI of 1 for 104 cells per 96 well
  • #KC30900: AdenoMag Starting Kit with Super Magnetic Plate + 200 µL of AdenoMag
  • #KC30996 : AdenoMag Starting Kit with plate 96 magnets + 200 µL of AdenoMag
  • #KC30600: ViroMag Triple Starting Kit with Super Magnetic plate + 100 µL of ViroMag, AdenoMag, ViroMag RL
  • #KC30696 : ViroMag Triple Starting Kit with plate 96 magnets + 100 µL of ViroMag, AdenoMag, ViroMag RL
Storage: +4°C
Shipping Conditions: Room temperature
  • This reagent needs to be used with a magnetic plate
CATALOG NUMBER UNIT SIZE
AM70100

100 µL
AM70200

200 µL
AM71000

1 mL
KC30900

AdenoMag Starting Kit
KC30996

AdenoMag Starting Kit plate 96 magnets
KC30600

ViroMag Triple Starting Kit
KC30696

ViroMag Triple Starting Kit with plate 96 magnets

 

Applications

  • Ideal for boosting your cell transduction with all adenoviral and AAV vectors
  • Suitable for all mammalian cells: cell lines, primary cells, hard-to-transfect & non-permissive cells

 RECOMMENDED FOR: Adenovirus and adeno-associated-virus transduction in vitro 

 

Results

AdenoMag Transfection results

Figure 1: Adenomag enhances transgene expression: beta-Galactosidase expression was determined in HEK-293, NIH-3T3, C6 and COS7 cell lines after 24H of infection with Ad-LacZ, Ad-LacZ with Polybrene (Ad-LacZ + Pb) or Ad-LacZ with 2 μL of AdenoMag (Ad-LacZ + AM).

AdenoMag Transduction results

Figure 2: AdenoMag enhances the number of infected cells: Representative images of HUVEC infected with Ad-GFP (MOI of 5) and either 0 (d), 0.5 (e) or 1 μL (f) of AdenoMag. Photos were taken under white field (a, b and c) and fluorescence (d, e and f) after 48 H infection.