PCR SuperMixes

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2×TransTaq® -T PCR SuperMix

TransTaq®-T PCR SuperMix is a ready-to-use mixture of TransTaq®-T DNA Polymerase, dNTPs and optimized buffer.

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Overview

TransTaq®-T PCR SuperMix is a ready-to-use mixture of TransTaq®-T DNA Polymerase, dNTPs and optimized buffer. The SuperMix is provided at 2× concentration and used at 1× concentration by adding template, primers and H2O. Efficiency of PCR products with “A” is equal to EasyTaq® DNA polymerase. It is more suitable for high fidelity TA cloning.
 

• TransTaq®-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 8 kb.
 

Applications
• Complex templates
• TA cloning
 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents
Component AS122-01/21 AS122-02/22
TransTaq®-T PCR SuperMix (-dye)/(+dye) 1 ml 5×1 ml
Nuclease-free Water 1 ml 5 ml