PCR SuperMixes

2×TransStart® GoldPfu PCR SuperMix (-dye) is a ready-to-use mixture of TransStart® GoldPfu DNA polymerase, dNTPs, and optimized reaction buffer showing high amplification efficiency, fast amplification, ultra high fidelity and high specificity. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and Nuclease-free Water. The amplified products with blunt ends can be directly cloned into pEASY®-Blunt vectors. The SuperMix without dye (-dye) cannot be directly loaded on agarose gel for electrophoresis.

Its PCR product is not suitable for polyacrylamide gel electrophoresis. 

 

• Reduced PCR operation time.

• Avoid contamination due to multi-step operation.

• 54-fold fidelity as compared to EasyTaq® DNA Polymerase.

• Amplification of genomic DNA fragment up to 15 kb.

• Amplification of plasmid DNA fragment up to 20 kb.

 

Applications

• Ultra High fidelity PCR

• Site-directed mutagenesis

• Blunt end cloning

• Complex templates

• GC/ AT rich templates

• Long fragment PCR

 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AS401-01	AS401-02	AS401-03
2×TransStart® GoldPfu PCR SuperMix (-dye)	1 ml	5×1 ml	15×1 ml
Nuclease-free Water	1 ml	5 ml	3×5 ml