PCR SuperMixes

TransStart® FastPfu PCR SuperMix is a ready-to-use mixture of TransStart® FastPfu DNA polymerase, dNTPs, and optimized  buffer, featuring high amplification efficiency, fast amplification speed, high fidelity and high specificity. The SuperMix is  provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2 O for amplification. The  amplified product of 2×TransStart® FastPfu PCR SuperMix is blunt-ended and can be cloned directly into pEASY®-Blunt  series of vectors. It can also be directly loaded on agarose gel for electrophoresis. If it is used for cloning, it needs to be purified  to remove the dye.  

Its PCR product is not suitable for polyacrylamide gel electrophoresis.

 

• Reduce PCR operation time. 

• Avoid contamination caused by the multi-step operation. 

• TransStart® FastPfu PCR SuperMix offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase. 

• Amplification of genomic DNA fragment up to 15 kb. 

• Amplification of plasmid DNA fragment up to 20 kb.

 

Applications
• High fidelity PCR 

• Site-directed mutagenesis  

• Blunt end cloning  

• Complex templates  

• Amplification of templates with high GC/AT content  

• Long fragment amplification

 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AS221-01	AS221-02
2×TransStart® FastPfu PCR SuperMix (-dye)/ (+dye)	1 ml	5×1 ml
Nuclease-free Water	1 ml	5 ml