PCR SuperMixes

TransStart® FastPfu Fly PCR SuperMix is a ready-to-use mixture of TransStart® FastPfu Fly DNA polymerase, dNTPs, and optimized buffer, featuring high amplification efficiency, fast amplification speed, high fidelity and high specificity. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H₂O for amplification. The amplified product of 2×TransStart® FastPfu Fly PCR SuperMix is blunt-ended and can be cloned directly into pEASY®-Blunt series of vectors.

 

•Reduce PCR operation time.

•Avoid contamination caused by multi-step operation.

•TransStart® FastPfu Fly PCR SuperMix offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.

•Amplification of genomic DNA fragment up to 15 kb.

•Amplification of plasmid DNA fragment up to 20 kb.

 

Applications

• Ultra high fidelity PCR 

• Site-directed mutagenesis 

• Blunt end cloning 

• Complex templates PCR

• GC/AT-rich templates PCR

• Long fragment amplification

 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AS231-01	AS231-02
2×TransStart® FastPfu Fly PCR SuperMix (-dye)	1 ml	5×1 ml
Nuclease-free Water	1 ml	5 ml